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Pichia pastoris, Nanobodies, methanol-free production system

Abstract

Pichia pastoris (syn. Komagataella phaffii) is one of the cornerstones of Sanofi’s microbial expression platform. The use of the methanol-inducibleexpression system for production was the golden standard for years, delivering high titers and unsurpassed quality. However, for large scale manufacturing, the use of methanol as toxic substance increases the complexity of HSE & safety requirements. Hence, Sanofi’s microbial expression platform is looking for an alternative system that is capable of matching the performance of methanol-based processes but without the drawbacks associated with the  use of methanol. Having a safe- and eco-friendly process design in mind, Sanofi is working on establishing a methanol-free expression platform for Nanobody® molecule production. Recent developments to optimize the methanol-free process have emphasized the potential of this new platform to  achieve commercially competitive productivity and product quality. Further progress in the field of strain-engineering and process design will aid to advance the Pichia-based production platform further.